MCQ on Chromatography – How Well Do You Know the Principles and Applications?

Chromatography is a powerful technique used for separating and analyzing complex mixtures. It is widely used in various fields such as pharmaceuticals, environmental sciences, food and beverage industries, and forensic science. In this article, we will discuss MCQ on chromatography that will help you to understand the basics of this technique.

MCQ on Chromatography : Attempt MCQ Now!

Below are the some of the MCQ on Chromatography to assist you in the preparation.

Q1 What is chromatography?

1. A technique used for analyzing the structure of molecules
2. A technique used for separating and analyzing complex mixtures
3. A technique used for detecting DNA sequences
4. A technique used for analyzing the physical properties of substances

Answer: B

Q2 Which of the following is not a type of chromatography?

1. Gas Chromatography
2. Paper Chromatography
3. Thin-layer Chromatography
4. Cellulose Chromatography

Answer: D

Q3 Which of the following statements is true about Gas Chromatography?

1. It is used for separating and analyzing complex mixtures of volatile compounds
2. It is used for separating and analyzing complex mixtures of non-volatile compounds
3. It is used for detecting DNA sequences
4. It is used for analyzing the physical properties of substances

Answer: A

Q4 Which of the following statements is true about High Performance Liquid Chromatography (HPLC)?

1. It is a type of gas chromatography
2. It is used for separating and analyzing complex mixtures of non-volatile compounds
3. It is used for detecting DNA sequences
4. It is used for analyzing the physical properties of substances

Answer: B

Q5 What is the stationary phase in chromatography?

1. The phase that is in motion
2. The phase that is in a fixed position
3. The phase that is used to detect compounds
4. The phase that is used for separation

Answer: B

Q6 What is the mobile phase in chromatography?

1. The phase that is in motion
2. The phase that is in a fixed position
3. The phase that is used to detect compounds
4. The phase that is used for separation

Answer: A

Q7 What is the principle of chromatography?

1. Separation of compounds based on their boiling point
2. Separation of compounds based on their density
3. Separation of compounds based on their polarity
4. Separation of compounds based on their molecular weight

Answer: C

Q8 Which of the following factors can affect the separation in chromatography?

1. Temperature
2. Flow rate
3. pH of the mobile phase
4. All of the above

Answer: D

Q9 Which of the following statements is true about Thin-layer Chromatography (TLC)?

1. It is used for separating and analyzing complex mixtures of non-volatile compounds
2. It is used for detecting DNA sequences
3. It is used for analyzing the physical properties of substances
4. It is a simple and inexpensive technique used for qualitative analysis

Answer: D

Q10 What is the detection method used in Gas Chromatography (GC)?

1. UV-Vis Spectrophotometry
2. Mass Spectrometry
3. Infrared Spectroscopy
4. Nuclear Magnetic Resonance Spectroscopy

Answer: B

Q11 Which of the following is a disadvantage of Paper Chromatography?

1. Low resolution
2. High cost
3. Long separation time
4. Low sensitivity

Answer: A

Q12 Chromatography is a technique used for:

1. a) Separation of a mixture of compounds
2. b) Analysis of a single compound
3. c) Both (a) and (b)
4. d) None of the above

Answer: a) Separation of a mixture of compounds

Q13 What is the principle of chromatography?

1. a) Separation of compounds based on their physical properties
2. b) Separation of compounds based on their chemical properties
3. c) Separation of compounds based on their size
4. d) Separation of compounds based on their mass

Answer: a) Separation of compounds based on their physical properties

Q14 Which of the following is not a type of chromatography?

1. a) Paper chromatography
2. b) Gas chromatography
3. c) Liquid chromatography
4. d) Nuclear magnetic resonance chromatography

Answer: d) Nuclear magnetic resonance chromatography

Q15 Which of the following is a stationary phase in gas chromatography?

1. a) Gas
2. b) Liquid
3. c) Solid
4. d) None of the above

Answer: c) Solid

Q16 Which of the following is a mobile phase in gas chromatography?

1. a) Gas
2. b) Liquid
3. c) Solid
4. d) None of the above

Answer: a) Gas

Q17 Which of the following is a stationary phase in liquid chromatography?

1. a) Gas
2. b) Liquid
3. c) Solid
4. d) None of the above

Answer: c) Solid

Q18 Which of the following is a mobile phase in liquid chromatography?

1. a) Gas
2. b) Liquid
3. c) Solid
4. d) None of the above

Answer: b) Liquid

Q19 Which type of chromatography separates compounds based on their charge?

1. a) Ion-exchange chromatography
2. b) Affinity chromatography
3. c) Size-exclusion chromatography
4. d) Reverse-phase chromatography

Answer: a) Ion-exchange chromatography

Q20 Which type of chromatography separates compounds based on their affinity for a specific ligand?

1. a) Ion-exchange chromatography
2. b) Affinity chromatography
3. c) Size-exclusion chromatography
4. d) Reverse-phase chromatography

Answer: b) Affinity chromatography

Q21 Which type of chromatography separates compounds based on their size?

1. a) Ion-exchange chromatography
2. b) Affinity chromatography
3. c) Size-exclusion chromatography
4. d) Reverse-phase chromatography

Answer: c) Size-exclusion chromatography

Q22 Which type of chromatography separates compounds based on their hydrophobicity?

1. a) Ion-exchange chromatography
2. b) Affinity chromatography
3. c) Size-exclusion chromatography
4. d) Reverse-phase chromatography

Answer: d) Reverse-phase chromatography

Q23 Which type of chromatography is commonly used for separating proteins?

1. a) Ion-exchange chromatography
2. b) Affinity chromatography
3. c) Size-exclusion chromatography
4. d) Reverse-phase chromatography

Answer: b) Affinity chromatography

Q24 What is the retention time in chromatography?

1. a) The time taken for a compound to reach the detector
2. b) The time taken for a compound to elute from the column
3. c) The time taken for a compound to dissolve in the mobile phase
4. d) The time taken for a compound to be injected onto the column

Answer: b) The time taken for a compound to elute from the column

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